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Hydrolysis is typically achieved under acid conditions. A standard procedure is hydrolysis with 6 M hydrochloric acid (24 hours, 110°C). Fragile amino acids, especially tryptophan and cysteine, will be partially destroyed. And then hydrolyzed samples (amino acid analysis service) are derivatized pre-column or post column for sensitive detection, separated by RP/SCX column. The use of internal and external standards of known amount is crucial for accurate quantification of each amino acid.
Equipped with automatic amino acid analyzer, Creative Proteomics can provide highly reproducible quantitative analysis of protein/peptide samples the customers submit, for estimation of amino acid composition of specific proteins, and purity determination.