Protein Phosphorylation Analysis is the most ordinarily contemplated region of post-translational adjustment since it assumes a crucial part in intracellular flag transduction and is included in managing cell cycle movement, separation, change, improvement, peptide hormone reaction, and adjustment. It has been evaluated that 33% of mammalian proteins might be phosphorylated and this change regularly assumes a key part in balancing protein work. Reversible protein phosphorylation, primarily on serine, threonine or tyrosine buildups, is a standout amongst the most essential and all around examined post-translational changes.